Fufu free essay sample

Fufu Fufu is a staple food, in other words, eaten in large amounts. It is made by boiling starchy food crops and pounding them into a big ball of dough. It is eaten by taking a small part of it with your fingers and dipping it into some soup or sauce. They eat it with light pepper soup, nut soup, groundnut soup, or other types of soup made with meat and vegetables. You are to then swallow it whole because chewing the ball of fufu is traditionally discouraged. We will write a custom essay sample on Fufu or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page It is made by boiling starchy food crops and pounding them into a big ball of dough. It is eaten by taking a small part of it with your fingers and dipping it into some soup or sauce. They eat it with light pepper soup, nut soup, groundnut soup, or other types of soup made with meat and vegetables. You are to then swallow it whole because chewing the ball of fufu is traditionally discouraged.

buy custom Oticon and Information Technology essay

buy custom Oticon and Information Technology essay Information technology is a significant facet in any organization, be it a multinational or a small business. It is also essential in society in general. Integrating information technology in any office set up means employment of computer software, hardware and processor-oriented information systems. Most of the modern organizations have incorporated information technology in their systems, for smooth running of the firms. Effective inclusion of information technology in the office, could lead to remarkable outcomes, though it could also have its demerits. The major limitation of information technology is seen to be the redundancy of managers and workers. There are however several organizations that have been effectively renovated with the incorporation of information technology, one such example being Oticon. Oticon and Information Technology For the main part, Oticon demonstrates a good paradigm of successful incorporation of computer-based technology in redesigning an organization positively. Oticon is an international producer of hearing aids with its headquarters based in Denmark. This company was on the verge of being rendered extinct, due to competition from an American company that was producing hearing aids that were preferable to the larger market. This was when the firm got a new chief executive officer, Lars Kolind, in 1988 who revamped Oticon. Bjorn-Andersen and Turner (1995) suggest that information technology was not the sole reason for the far-reaching changes that took place at Oticon, but it made the metamorphosis possible and pragmatic. Information technology was applied by Oticon to make the work environment more efficient and mobility of workers was increased. Information was also afforded to workers at more convenient places and time as compared to the inconveniences experienced with the office syste m. Role of Information Technology in Organizations Information technology in an organization takes on a facilitative position rather than the primary role. It enables a company to undertake restructuring of its management and day to day workings as Oticon did. This kind of technology is used in creation of new business ideas, inventing novel methods of working and solving organizational hitches. Computer software for example, is used to design an easier and more effective way of coordinating the affairs of the company. For instance there can be accounting software to help in book keeping and inventory management. The Point of Sale (POS) system, for example ensures that whenever an item is sold from the stock in a business then the reduction in stock is reflected in the system. Applying information technology in organizations is manifested through the use of e-mails to communicate in an office. More recently there has been an increase of businesses and even big organizations using social media sites, such as Facebook, to advertise and market their products. Organizations also design websites where potential investors and clients can access information on the firm. Power point presentations are also used in company meetings to make a task more effectual and easy to understand. Most organizations have also resolved to use digital versions of files instead of filing cabinets filling a whole room with documents and files. In this way a huge amount of historical data can be stored and easily retrieved. Organizations also use computers to store the database of their employees. This is also useful for potential employees who send their job applications through the internet. Organizations also use information technology to reach out to their customers and receive feedback on products as well as to improve on customer care. Research can also be done on the internet to ensure that an organization keeps up with modern products and market trends. The Case of Johnsonville Sausage LLC Johnsonville Sausage LLC is an American company based in Wisconsin which mainly deals with manufacturing of meat products. This is one of the big companies that has effectively used technological strategies to sway global markets and consumer tastes. In 2003, the company mapped out an information technology plan to set up an all inclusive SAP policy. This meant that the firm would advance its visibility and be able to exploit more sources of raw materials, end products and what the customers require most. Johnsonville Sausage LLC utilized the Cisco Aironet 350 Series and set up a wireless local area network to follow up on the movement of its manufactured goods (Cisco Systems Inc. 2001). The company has also used other Cisco products such as firewalls to protect confidential company information and money, as well as routers and hubs. Johnsonville Sausage has also made use of wireless machinery to connect computers which are on the floor to the network. This is in turn used to check on the inventory as it stands at that particular time. All these illustrations demonstrate that Johnsonville Sausage LLC has indeed transformed due to information technology advancements. I would like to work in such an environment as this would mean that work gets done faster as information is available and is conveniently retrieved. In such an environment there is advanced technology and safeguards and the business is expanding meaning there is job security and better salaries too. Conclusion This paper has illustrated that information technology if effectively utilized and integrated by an organization can lead to a remarkable transformation. It is important to note that the role of information systems and technology is merely enabling or facilitative. To obtain optimum organizational transformation, a firm still has to do much more, for instance building up the main competencies and careers of the organization. Buy custom Oticon and Information Technology essay

Films as Essential Tools of National Historical Analysis Research Paper

Films as Essential Tools of National Historical Analysis - Research Paper Example Cinematic and narrative elements of movies together with other stylistic devices help to bring out the intended meaning or outcome. Modern filmmakers have creatively used various aspects that show events that depict historical growth of particular nations. These films show the reasons why certain historical aspects have faded away or have remained and why they hold particular importance to those nations. Au Revoir les Enfantes also known as Goodbye Children is about a French boarding school that is under the administration of priests (Everett 49). The school seems to be a place of protection where people enjoyed peace and harmony until a new student gets into the school. The new student was allocated a room, which he could share with a student who was top in his class. Despite the fact that they became rivals at their first contact, they later form an inseparable bond linked by a shared secret. They became friends one night when they got lost in the woods and are rescued by German so ldiers. The soldiers wrapped them in blankets and drove them back to school. The film was written and directed by Louis Malle. It was produced in the year 1987 (Everett 49). The movie is based on an event that happened in January 1944. Louis Malle was twelve years old when the incident happened. At that time, he was attending a Jesuit boarding school along Fountainebleau. After the Christmas holiday had ended, schools were back with normal classes and other operations. In the middle of the scholastic year, three new students joined the school one of whom became a rival and competitor with Mall. Malle used to top the class in scholastic domain. After several weeks in school, Germans arrested the young boy who competed with Malle together with the other newcomers. Julien did not know about the true identity of jean but tried as much as he could and learnt that Jean was a pseudonym. The headmaster of the school also disappeared at the same time. The three boys were of Jewish decent. Th e convent school in Au revoir les enfants, on the other hand, is an elite institution for wealthy children, and it attempts to insulate itself from events outside its walls.   This is also a familiar trope in Holocaust/Occupation films... the wealthy elite who go into denial and/or lie about what's going on around them in order to hang onto not just wealth and power, but also customs, tradition, and civility. As the film documents, films can be used to show important historical events that took place at a certain period in time. This film captures the events that took place during the Second World War and their impacts. It is a fiction film that was created from the memories from a journalist’s conscience (Aitken 207). It is a story about France during the Second World War. It is essential to note that the young boy, Jean Bonnet was different from the other students in the school. He had a curly hair and did not eat pork. However, the secret about his decent becomes an open secret when everyone knows that he is Jewish. The catholic priests who are the administrators of the school admitted the Jewish boys as an act of charity because the boys lived as pseudonyms because they did not know the whereabouts of their parents (Aitken 207). Malle’s films clearly show the use of the narrative trope of class, morality, and opportunity during crisis. For instance,  Lucien Lacombe is a working-class farm boy who is able to advance himself during the Vichy crisis,

Analysis and evaluation of an Australian resource company Essay - 1

Analysis and evaluation of an Australian resource company - Essay Example But its mining operations only started in 2009 which initially produced 2,465 ounces of gold and 1,468 oz of silver amounting to $3,378 million (A1, 2011). It claimed that it’s producing gold at average of 450 gold per week and 400 of silver (A1, 2011). The companies have built its facilities with contracted team of engineers to enhance its working environment as it worked with mining contractor Watpac that is gauging its credibility on Brightstar project to get into Western Australian gold mining circles (A1, 2011). It also used its facilities to accommodate workers and contractors, some of whom operates on fly-in, fly-out schedule. Facilities installed are modern communications, a laboratory and a gold room (A1, 2011). A1 targeted to open four minefields they coded as Alpha, Beta, delta and Epsilon. The sum total area is 1,559,054t at 4.2g/t ore (A1, 2011). The company perceived at extracting about 4,915, 879 grams of and gold (in situ) and hope to estimate to get 150,164 ounces of gold (A1, 2011). The company has transparent financial policies, accounting of renumeration, and stocks shares (A1, 2011). It accorded independence of auditor in the evaluation and accounting of its finances. However, it is interesting to note that its consolidated financial report reflected huge losses of $5,900,505 in 2010 and $4,282,979 in 2009 compared to its declared income/revenue of $ 2,769,155 (2010) and % $ 199,062 in 2009 (A1, 2011). The management further declared that they suffer financial setback with $6,047,861 deficiency of working capital (A1, 2011). It is further affirmed that the company need to gather more capital to fund its consolidated operations, exploration, and assets management (A1, 2011). The management hope to undertake aggressive exploration to extract in an open-pit method the mineral reserves in Australia of about 20M oz in Laverport region and in other significant regions. Last year, 2010, A1 sold its northern gold prospect to Regis Mining,

A World of Art Essay Example | Topics and Well Written Essays - 1250 words - 1

A World of Art - Essay Example The other the triangle between Mary and Jesus represents the cup of the last grail. Look out the windows, one can see the country side which is not congruent with the subject matter. He uses the technique of Chiaroscuro. It is dark in the room and bright outside. It is interesting to note that he used the same country side in his Mona Lisa painting which he completed in 1503. The colors used are green and orange(brown). There is symmetry with the large rolls on the table. Important paintings Perspectival study of the Adoration of the Magi: (Art Authority) Pen and ink, traces of silver point and white on paper. Fascinating to see his study in perspective of texture, the use of Chiaroscuro, the use of sienna to show white, the three dimensional by the fact that we can see the thickness of the stairs and the pillars. the lines showing how everything is done in perfect mathematical dimensions then The Adoration of the Magi: (Art Authority) oil on panel. The oil thing which remains from t he perspective is the stairs and the pillars but they are quite distant. It was as though he used the study to do several painting. The texture is very primitive for a Leonardo da Vinci. The trees are two dimensional and Mary is in one color. Perhaps it is a question of aging. The people are half real. Titian, The Assumption of the Virgin: Oil on wood. A perfect study in vertical perception there is an incredible sense of movement, towards the heavens. In analyzing the lines, there are the two triangles with two parallel lines. The texture of the fluffiness of the clouds but also the heaviness the angles have of carrying Mary Profane Love: a perfect contract between the Assumption and this perfect technique of Chiaroscuro. There is the light shining on her face and her hand. She is also looking at someone but we do not see him either. There is also the mirror which shows you something that the person in the painting doesn't see. This is a technique which began with Leonardo da Vinci . The texture of her clothes is magnificence Van Gogh, Starry Night : oil on canvas. It is not polychromatic though he does use a great deal of blue. He avoided using red. He uses hatching in the modern sense of the word. His brush makes short rapid strokes of the same color. The texture has very little dimension to it other than a little bit of the town. I would not call it two dimensional. Road with Pollarded Willows and A Man with a Broom (Art Authority) is from carbon, ink, and watercolor on paper. His texture is three dimensional. You can see the end of the road but you see the beginning of the impressionist school as the road slightly bends to the right. As he advanced in his training, he mixed different techniques each time of pencil and ink, or pencil, ink and water color to cite an example. He showed his being influenced from different schools. He painted 37 self portraits. It is documents that none of them look directly at the viewer but the Self Portrait 1887 looks straig ht on. His self portrait in the straw hat looks like a modern version of hatching. Jasper Johns, Numbers in Color: uses the technique of pattern and polychromatic. He called his paintings of clusters "crosshatchings (Johns) Grey numbers (Art Authority) is the same technique of patter without color Andrea Mantegna, The Lamentation over The Dead Christ 1494: tempera on canvas. The texture is three dimensional. He uses lines in one angle to gain depth to take away from the size of his feet. There is

Chromatographic Separation of Hemoglobin from Phenol Red

Chromatographic Separation of Hemoglobin from Phenol Red Syed Ibrahim Chromatographic Separation of Hemoglobin from Phenol Red by Gel Filtration Introduction From catalysis to transport, proteins take part in countless cellular processes (Nelson Cox, 2008). However, due to their small size, proteins can be challenging to work with in the lab. To overcome this hurdle, scientists have developed many fractionation techniques including centrifugation, precipitation, dialysis, and chromatography (Alberts et al., 2008). Chromatography is one of the most versatile laboratory techniques as it is used in many fields of study (Partridge, 1952; Silva et al., 2014). There are several types of chromatography, and each separates molecules based on different properties. Size-exclusion gel filtration is a type of column chromatography which resolves molecules based on their size (Duong Gabelli, 2013). In the column, there is a cross-linked polymer matrix with pores of a predetermined size (Scott, 2003). Small molecules are more likely to spend time in the pore, so they migrate slower and thus elute in later fractions (Nelson Cox, 2008). Conversely, larger molecules are less likely to enter the pore, so they migrate faster and thus elute in earlier fractions (de Jong et al., 2015). There are numerous types of column matrices, such as Sephadex G (composed of dextran and epichlorohydrin) and Sepharose (consisting of agarose) (GE, 2015). Each matrix has different sized pores and thus different ranges of molecular sizes that it can separate, called fractionation ranges (de Jong et al., 2015). In this lab, Sephadex G-25, which has a fractionation range of 1,000-5,000Da, was the column matrix (de Jong et al., 2015). Hemoglobin and phenol red were also used. Hemoglobin is a protein found primarily found in mammalian red blood cells, and is used to transport oxygen from an organism’s lungs to its tissues (Seeley, 2014). Phenol red is a chemical compound that changes color depending on the pH of the chemical environment, and so it is typically used as a pH indicator (Klein, 2012). Hemoglobin has a molecular weight of 64,500Da while phenol red has a molecular weight of 354.38Da (de Jong et al., 2015). It was expected that hemoglobin would be completely excluded from the pores of the Sephadex G-25 resin, allowing it to elute in early fractions. As well, it was expected that phenol red would be completely included in the gel, making it elute in later fractions. Purpose The goal of this lab was to separate a mixture containing hemoglobin and phenol red using size-exclusion gel filtration. As well, the aim was to compare the standard and literature wavelengths of maximum absorption of hemoglobin and phenol red. Results The results from this experiment are shown in Table 1 and Table 2. Table 1. Fraction volumes from size-exclusion gel filtration using Sephadex G25. The size-exclusion gel filtration separated a mixture of hemoglobin and phenol red. In theory, fraction A contained void volume, fraction B contained hemoglobin, fraction C contained the buffer only, and fraction D contained phenol red. Table 2. Peak absorbance and wavelength of standard hemoglobin and phenol red. Using a Beckman spectrophotometer, the wavelength profile between 380nm and 600nm of two standard samples was determined. The standard hemoglobin sample contained 250ÃŽ ¼L of 0.8% hemoglobin, while the standard phenol red sample consisted of 20ÃŽ ¼L of 0.2% phenol red. Based on the graph, the maximum absorbance values and the corresponding wavelengths were determined. Questions and Research Question 1: Search Engine: Web of Science Search Terms: hemoglobin spectroscopy Reference: DiFeo, T. J., Addison, A. W., Stephanos, J. J. (1990). Kinetic and spectroscopic studies of haemoglobin and myoglobin from Urechis caupo. Distal residue effects. Biochem. J, 269, 739-747. Search Engine: Web of Science Search Terms: hemoglobin Soret band absorptivity (sorted by relevance) Reference: Akuwudike, A. R., Chikezie, P. C., Chilaka, F. C. (2010). Absorption spectra of normal adult and sickle cell haemoglobins treated with hydrogen peroxide at two pH values. Adv. Biomed. Res, 1, 55-60. Search Engine: Google Search Terms: phenol red maximum wavelength absorption Reference: LobaChemie. (2015). Phenol Red. Laboratory Reagents and Fine Chemicals. Retrieved February 21, 2015, from http://www.lobachemie.com/pH-Indicator-05180/PHENOL-RED-CASNO-143-74-8.aspx Based on the graph from Blackboard, the wavelengths of maximum absorbance (ÃŽ »max) are shown in Table 2. For the standard hemoglobin sample ÃŽ »max = 410nm, and for the standard phenol red sample ÃŽ »max = 560nm. Based on the literature, hemoglobin and related proteins are known to give three bands of maximum absorption: ÃŽ ±-band, ÃŽ ²-band, and Soret band (DiFeo et al., 1990). For normal adult hemoglobin (HgA), the wavelength of maximum absorbance in the Soret band is ÃŽ »max = 415nm (Akuwudike et al., 2010). The wavelengths of maximum absorbance of phenol red changes based on pH. At a pH of 8.2, phenol red has ÃŽ »max = 557-560nm while at a pH of 6, phenol red has ÃŽ »max = 430-435 (LobaChemie, 2015). This change in maximum absorbance with respect to pH is understandable because phenol red is a common pH indicator, meaning it changes color based on pH (Nelson Cox, 2008). The literature and experimental wavelengths of maximum absorbance are summarized in Table 3. The experimental and literature values were fairly close, and their variance may be accounted for due to difference in the buffer and pH of the solvent. Table 3. Literature and experimental wavelengths of maximum absorbance. The experimental wavelengths of maximum absorbance (ÃŽ »max) were obtained from Table 2. The literature ÃŽ »max of hemoglobin (HgA) was based on Akuwudike et al (2010). The literature ÃŽ »max of phenol red at pH = 8.2 was based on LobaChemie (2015). Question 2: Based on the data collected in this experiment, it is impossible to determine whether the eluted hemoglobin is free of phenol red. To determine its purity, spectroscopy of the eluted hemoglobin (fraction B) would need to be conducted in order to draw a wavelength-vs-absorbance graph between 380nm and 600nm (similar to the one posted on Blackboard). If the eluted hemoglobin curve has only one peak (ÃŽ »max = 410-415nm), it contains only hemoglobin. However, it the curve has two peaks (ÃŽ »max = 410-415nm and ÃŽ »max = 557-5605nm), then the eluted fraction contains both hemoglobin and phenol red. Question 3: Search Engine: Web of Science Search Terms: size-exclusion gel filtration (sorted by relevance) Reference: Wang, Q., et al. (2005). Molecular characterisation of soybean polysaccharides: an approach by size exclusion chromatography, dynamic and static light scattering methods. Carbohydrate research, 340(17), 2637-2644. In this experiment, size-exclusion gel filtration was carried out, so hemoglobin and phenol red were separated based on size or more quantitatively based on molecular weight (Nelson Cox, 2008; Wang et al., 2005). Smaller molecules, such as phenol red, spend more time in the pores and are eluted in later fractions (Nelson Cox, 2008). Conversely, larger molecules molecules, such as hemoglobin, spend almost no time in the pores and are eluted in earlier fractions (Nelson Cox, 2008). Question 4: Search Engine: Web of Science Search Terms: chromatography theory (sorted by relevance) Reference: Lee, W. C., Tsai, G. J., Tsao, G. T. (1993). Analysis of chromatography by plate theory. Separations Technology, 3(4), 178-197. Search Engine: PubMed Search Terms: chromatography theory column length (sorted by relevance) Reference: Bedani, F., et al. (2006). A theoretical basis for parameter selection and instrument design in comprehensive size-exclusion chromatographyÃâ€" liquid chromatography. Journal of Chromatography A, 1133(1), 126-134. Based on the plate theory of chromatography, the resolution (R) is influenced by numerous factors including the plate number (N) (Lee et al., 1993). The plate number is affected by many elements of the column including column length and inner column radius (Bedani et al., 2006). Based on mathematical relationships, it has been determined that longer columns lead to increased resolution (Lee et al., 1993; Nelson Cox, 2008). Thus longer chromatography columns will separate molecules better than shorter columns. Question 5: In order to separate ÃŽ ²1,3-galactosidase (75,000Da) and ÃŽ ²1,2-xylosidase (MW 100,000Da) the Sephadex G-100 resin would be ideal. This is because the molecular weight of both enzymes is within the fractionation range of this resin (4,000-150,000Da) (de Jong et al., 2015). The heavier protein (ÃŽ ²1,2-xylosidase) will elute before the lighter protein (ÃŽ ²1,3-galactosidase). Question 6: The fractionation range of Sephadex G-100 is 4,000-150,000Da (de Jong et al., 2015). Alcohol dehydrogenase (MW 140,000Da) falls within this range, while aldehyde dehydrogenase (MW 200,000Da) is above the range. Since both enzymes are not on the same extreme of the fractionation range, aldehyde dehydrogenase will elute very quickly and alcohol dehydrogenase will elute shortly after. Thus, Sephadex G-100 can be used to resolve alcohol dehydrogenase and aldehyde dehydrogenase. However, it would be ideal to use Sephadex G-200 because the molecular weight of both enzymes would be within the fractionation range of 5,000-600,000Da (de Jong et al., 2015). Question 7: Search Engine: PubMed Search Terms: â€Å"fast protein liquid chromatography† (sorted by relevance) Reference: Sheehan, D., O’Sullivan, S. (2004). Fast protein liquid chromatography. In Protein purification protocols (pp. 253-258). Humana Press. Search Engine: PubMed Search Terms: â€Å"fast protein liquid chromatography† (sorted by relevance) Reference: Goke, B., Keim, V. (1992). HPLC and FPLC. Recent progress in the use of automated chromatography systems for resolution of pancreatic secretory proteins. International Journal of Pancreatology, 11(2), 109-116. Search Engine: PubMed Search Terms: â€Å"fast protein liquid chromatography† (sorted by relevance) Reference: Lavanya, G., et al. (2011). Protein Chromatography. J Biomed Sci and Res, 3(3), 424-438. Search Engine: Google Search Terms: fast protein liquid chromatography Reference: Bio-Rad. (2015). Fast Protein Liquid Chromatography. Bio-Rad Life Science Research.Retrieved February 22, 2015, from http://www.bio-rad.com/en-ca/applications-technologies/fast-protein-liquid-chromatography Search Engine: PubMed Search Terms: fast protein liquid chromatography use Reference: Verbeke, K., Verbruggen, A. (1996). Usefulness of fast protein liquid chromatography as an alternative to high performance liquid chromatography of 99m Tc-labelled human serum albumin preparations. Journal of pharmaceutical and biomedical analysis, 14(8), 1209-1213. Fast protein liquid chromatography (FPLC) is an automated chromatography technique that uses columns with a small inner diameter (Goke Keim, 1992; Sheehan O’Sullivan, 2004). By means of pumps and valves, the FPLC system regulates how fast the liquid mobile phase moves down the agarose stationary phase a constant, and maintains a constant flow rate (Lavanya et al., 2011). As well, based on the readings of the UV detector and conductivity meter, the FPLC system is able to control how and when the eluted fractions should be separated (Bio-Rad, 2015). There are variations of FPLC that allow proteins to separated base on size, charge or hydrophobicity (Verbeke Verbruggen, 1996). FPLC, as its name suggests is quite useful in analyzing and purifying protein mixtures (Lavanya et al., 2011). For example, FPLC has been used to identify zinc-containing proteins in rat saliva; and it has also been used to separate and analyze pancreatic juice in order to examine disease-causing proteins (Lavanya et al., 2011). Conclusion In this lab, a mixture of hemoglobin and phenol red was resolved using sizeexclusion gel filtration. Since Sephadex G-25 (fractionation range 1,000-4,000Da) was used as the column matrix, it was expected that hemoglobin would be completely excluded from the matrix pores, allowing it to elute in early fractions. As well, it was anticipated that phenol red would be completely included in the gel, making it elute in later fractions. The hemoglobin/phenol red mixture was separated into four fractions and their respected volumes are shown in Table 1. In theory, fraction A contained void volume, fraction B contained hemoglobin, fraction C contained the buffer only, and fraction D contained phenol red. Due to time and budget constraints, the absorbance of the eluted fractions was not analyzed. However based on an absorbance-vs-wavelength graph, the wavelengths of maximum absorbance (ÃŽ »max) for standard hemoglobin (ÃŽ »max = 410nm) and standard phenol red (ÃŽ »max = 560nm) were determined, as described in Table 2. These values were also compared to literature values, as shown in Table 3. Normal adult hemoglobin (HgA) is known to have maximum absorbance at ÃŽ »max = 415nm, while phenol red is known to have ÃŽ »max = 557-560nm. The experimental and literature values were fairly close to one another, and their slight variance may be accounted for due to difference in the buffer used and pH of the solvent. For further research, spectroscopy of the eluted hemoglobin (fraction B) would need to be conducted in order to draw a wavelength-vs-absorbance graph between 380nm and 600nm. This would help to clarify whether the eluted hemoglobin is free of phenol red. References Akuwudike, A. R., Chikezie, P. C., Chilaka, F. C. (2010). Absorption spectra of normal adult and sickle cell haemoglobins treated with hydrogen peroxide at two pH values. Adv. Biomed. Res, 1, 55-60. Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2008).Molecular Biology of the Cell(5th ed.). New York: Garland Science. Bedani, F., et al. (2006). A theoretical basis for parameter selection and instrument design in comprehensive size-exclusion chromatographyÃâ€" liquid chromatography. Journal of Chromatography A, 1133(1), 126-134. Bio-Rad. (2015). Fast Protein Liquid Chromatography. Bio-Rad Life Science Research. Retrieved February 22, 2015, from http://www.bio-rad.com/en-ca/applications-technologies/fast-protein-liquid-chromatography de Jong, M., Botelho, R., Antonescu, C., Wakarchuk, W. (2015).Laboratory Manual: Biochemistry- BCH261(Winter 2015 ed.). Toronto: Ryerson University. DiFeo, T. J., Addison, A. W., Stephanos, J. J. (1990). Kinetic and spectroscopic studies of haemoglobin and myoglobin from Urechis caupo. Distal residue effects. Biochem. J, 269, 739-747. Duong-Ly, K. C., Gabelli, S. B. (2013). Gel filtration chromatography (size exclusion chromatography) of proteins. Methods in enzymology, 541, 105-114. GE. (2015). Product Catalog. GE Healthcare Life Sciences. Retrieved February 21, 2015, from http://www.gelifesciences.com/webapp/wcs/stores/servlet/catalog/en/ GELifeSciences/products/products_by_technology/ Goke, B., Keim, V. (1992). HPLC and FPLC. Recent progress in the use of automated chromatography systems for resolution of pancreatic secretory proteins. International Journal of Pancreatology, 11(2), 109-116. Klein, D. R. (2012). Organic chemistry (2nd ed.). Hoboken, N.J.: John Wiley. Lavanya, G., et al. (2011). Protein Chromatography. J Biomed Sci and Res, 3(3), 424-438. Lee, W. C., Tsai, G. J., Tsao, G. T. (1993). Analysis of chromatography by plate theory. Separations Technology, 3(4), 178-197. LobaChemie. (2015). Phenol Red. Laboratory Reagents and Fine Chemicals. Retrieved February 21, 2015, from http://www.lobachemie.com/pH-Indicator-05180/PHENOL-RED-CASNO-143-74-8.aspx Nelson, D., Cox, M. (2008). Lehningers Principles of Biochemistry (5th ed.). New York, N.Y.: WH Freeman and Company. Partridge, M. W. (1952). Chromatography and its applications in pharmacy. Journal of Pharmacy and Pharmacology, 4(1), 217-230. Scott, R. P. (2003). Principles and practice of chromatography. Chrom-Ed Book Series, 1. Seeley, R. (2014). Seeleys Anatomy Physiology (10th ed.). New York, NY: McGraw-Hill. Sheehan, D., O’Sullivan, S. (2004). Fast protein liquid chromatography. In Protein purification protocols (pp. 253-258). Humana Press. Silva, J., Teodoro, J., Afonso, R., Aquino, S., Augusti, R. (2014). Photolysis and photocatalysis of ibuprofen in aqueous medium: characterization of byà ¢Ã¢â€š ¬Ã‚ products via liquid chromatography coupled to highà ¢Ã¢â€š ¬Ã‚ resolution mass spectrometry and assessment of their toxicities against Artemia Salina. Journal of Mass Spectrometry, 49(2), 145-153. Verbeke, K., Verbruggen, A. (1996). Usefulness of fast protein liquid chromatography as an alternative to high performance liquid chromatography of 99m Tc-labelled human serum albumin preparations. Journal of pharmaceutical and biomedical analysis, 14(8), 1209-1213. Wang, Q., et al. (2005). Molecular characterisation of soybean polysaccharides: an approach by size exclusion chromatography, dynamic and static light scattering methods. Carbohydrate research, 340(17), 2637-2644.

The Scarlet Letter 10 :: essays research papers

'Among many morals which press upon us from the poor minister's miserable experience, we put only this into a sentence: Be true! Be true! Be true! Show freely to the world, if not your worst, yet some trait whereby the worst may be inferred!';(Ch.24: 236) Hawthorne expresses the purpose of writing this novel in that short sentence. He creates characters who have sin and disguise these sins for their own salvation. Slowly these sins evolve the characters, it strengthens Hester, humanizes Dimmesdale, and turns Chillingworth into a demon. The story is Hawthorne's depiction of the effects of sin on the hearts and minds of humanity during the Puritan society through the characters Hester, Dimmesdale, and Chillingworth. Hester's sin is that her passion and love were of more importance to her than the Puritan moral code, but she learns the error of her ways and slowly regains the adoration of the community. For instance, 'What we did had a consecration of its own. We felt it so! We said so to each other! Hast thou forgotten it?';(Ch.17: 179). Hester fully acknowledges her guilt and displays it with pride to the world. This was obvious by the way she displays the scarlet letter with elaborate designs showing that she is proud. Furthermore, she does not want to live a life of lies anymore when she states 'forgive me! In all things else, I have striven to be true! Truth was the one virtue which I might have held fast, and did hold fast, through all extremity save when thy good--the life--they fame--were put in question! Then I consented a deception. But a lie is never good, even though death threaten the other side!';(Ch.17: 177). Hester learns from her sin, and grows strong, a direct result from her pu nishment. The scarlet letter 'A' was as if a blessing to Hester changing her into an honest person with good virtues. Fittingly, she chooses to stay in Boston with Pearl although Hawthorne admits, ';…that this woman should still call that place her home, where, and where only, she must needs be the type of shame';(Ch.5: 73). She is trying to stay and face her consequences instead of running in the other direction. Most people would leave a town where they are looked upon as trash the scum of society. Finally, the colonists come to think of the scarlet letter as '…the cross on a nun's bosom';(Ch.